Composite

Part:BBa_I716304:Design

Designed by: vincent parker   Group: iGEM07_Berkeley_UC   (2007-07-24)


OtsA-TT


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1591
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1591
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1576
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1591
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1591
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2678


Design Notes

Digest Bca1144-I716301 (EcoRI, BamHI 2063bp, 1440bp S) Sub into pBca9145-Bca1092 (EcoRI, BglII 2198bp, 9bp L) Product is Bca1144-I716304



Source


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.

References